首页|Identification and functional characterization of chemosensory genes in olfactory and taste organs of Spodoptera litura(Lepidoptera:Noctuidae)
Identification and functional characterization of chemosensory genes in olfactory and taste organs of Spodoptera litura(Lepidoptera:Noctuidae)
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The tobacco cutworm Spodoptera litura is one of the most destructive polyphagous crop pests.Olfaction and taste play a crucial role in its host plant selection and sexual communication,but the expression profile of chemosensory genes remains un-clear.In this study,we identified 185 chemosensory genes from 7 organs in S.litura by transcriptome sequencing,of which 72 genes were published for the first time,includ-ing 27 odorant receptors(ORs),26 gustatory receptors(GRs),1 ionotropic receptor(IR),16 odorant-binding proteins(OBPs),and 2 chemosensory proteins(CSPs).Phylogenetic analyses revealed that ORs,IRs,OBPs,and sensory neuron membrane proteins(SNMPs)were mainly expressed in antennae and sequence-conserved among Noctuidae species.The most differentially expressed genes(DEGs)between sexes were ORs and OBPs,and no DEGs were found in GRs.GR transcripts were enriched in proboscis,and the expres-sion of sugar receptors was the highest.Carbon dioxide receptors,sugar receptor-SliuGR6,and bitter GRs-SlituGR43 and SlituGR66 had higher sequence identities between Noctu-idae species.CSPs were broadly expressed in various organs,and SlituCSP13 was a DEG in adult antennae.The functional analysis in the Drosophila OR67d expression system found that SlituOR50,a receptor highly expressed in female antennae,is selectively tuned to farnesyl acetate.The results provide a solid foundation for understanding the molec-ular mechanisms by which chemosensory genes operate to elicit behavioral responses in polyphagous insects.
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