首页|An aquaporin and an aquaglyceroporin have roles in low temperature adaptation of mosquitoes(Anopheles sinensis)

An aquaporin and an aquaglyceroporin have roles in low temperature adaptation of mosquitoes(Anopheles sinensis)

扫码查看
原文链接
  • 万方数据
Mosquitoes(Anopheles sinensis),widely geographically distributed in Asia in-cluding China,are the primary vector of the malaria parasite Plasmodium vivax and other parasitic diseases such as Malayan filariasis.An.sinensis can survive through low winter temperatures.Aquaporin channels are found in all life forms,where they facilitate envi-ronmental adaptation by allowing rapid trans-cellular movement of water(classical aqua-porins)or water and solutes such as glycerol(aquaglyceroporins).Here,we identified and characterized 2 aquaporin(AQP)homologs in An.sinensis:AsAQP2(An.sinensis aquaglyceroporin)and AsAQP4(An.sinensis aquaporin).When expressed in frog(Xeno-pus laevis)oocytes,AsAQP2 transported water,glycerol,and urea;AsAQP4 transported only water.Water permeation through AsAQP2 and AsAQP4 was inhibited by mercuric chloride.AsAQP2 expression was slightly higher in adult female mosquitoes than in males,and AsAQP4 expression was significantly higher in adult males.The 2 AsAQPs were highly expressed in Malpighian tubules and midgut.AsAQP2 and AsAQP4 expression was up-regulated by blood feeding compared with sugar feeding.At freezing point(0 ℃),the AsAQP4 expression level increased and An.sinensis survival time reduced compared with those at normal temperature(26 ℃).At low temperature(8 ℃),the AsAQP2 and AsAQP4 expression levels decreased and survival time was significantly longer compared with those at 26 ℃.These results suggest that AsAQP2 and AsAQP4 have roles in water homeostasis during blood digestion and in low temperature adaptation of A.sinensis.To-gether,our results show that the 2 AQPs are important for mosquito diuresis after blood feeding and when exposed to low temperatures.

aquaporinaquaglyceroporinAnopheles sinensisblood digestionlow tem-perature adaption

Yong-Qiao Zhao、Yi-Ying Tang、Ju-Ping Hu、Yu-Zheng Huang、Kai Wan、Mei-Hua Zhang、Ju-Lin Li、Guo-Ding Zhu、Jian-Xia Tang

展开 >

National Health Commission Key Laboratory of Parasitic Disease Control and Prevention,Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology,Jiangsu Provincial Medical Key laboratory,Jiangsu Institute of Parasitic Diseases,Wuxi,Jiangsu,China

School of Public Health,Nanjing Medical University,Nanjing,Jiangsu,China

Wuxi School of Medicine,Jiangnan University,Wuxi,Jiangsu,China

2024

10.1111/1744-7917.13359

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(6)