首页|The symbiont Wolbachia alleviates pesticide susceptibility in the two-spotted spider mite Tetranychus urticae through enhanced host detoxification pathways
The symbiont Wolbachia alleviates pesticide susceptibility in the two-spotted spider mite Tetranychus urticae through enhanced host detoxification pathways
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万方数据
维普
The two-spotted spider mite(Tetranychus urticae)is one of the most well-known pesticide-resistant agricultural pests,with resistance often attributed to changes such as target-site mutations and detoxification activation.Recent studies show that pes-ticide resistance can also be influenced by symbionts,but their involvement in this pro-cess in spider mites remains uncertain.Here,we found that infection with Wolbachia,a well-known bacterial reproductive manipulator,significantly increased mite survival after exposure to the insecticides abamectin,cyflumetofen,and pyridaben.Wolbachia-infected(WI)mites showed higher expression of detoxification genes such as P450,glutathione-S-transferase(GST),ABC transporters,and carboxyl/cholinesterases.RNA interference experiments confirmed the role of the two above-mentioned detoxification genes,TuCYP392D2 and TuGSTd05,in pesticide resistance.Increased GST activities were also observed in abamectin-treated WI mites.In addition,when wild populations were treated with abamectin,WI mites generally showed better survival than uninfected mites.However,genetically homogeneous mites with different Wolbachia strains showed simi-lar survival.Finally,abamectin treatment increased Wolbachia abundance without altering the mite's bacterial community.This finding highlights the role of Wolbachia in orches-trating pesticide resistance by modulating host detoxification.By unraveling the intricate interplay between symbionts and pesticide resistance,our study lays the groundwork for pioneering strategies to combat agricultural pests.
万方数据
维普
