首页|Using kin discrimination to construct synthetic microbial communities of Bacillus subtilis strains impacts the growth of black soldier fly larvae

Using kin discrimination to construct synthetic microbial communities of Bacillus subtilis strains impacts the growth of black soldier fly larvae

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Using synthetic microbial communities to promote host growth is an effec-tive approach.However,the construction of such communities lacks theoretical guidance.Kin discrimination is an effective means by which strains can recognize themselves from non-self,and construct competitive microbial communities to produce more secondary metabolites.However,the construction of cooperative communities benefits from the widespread use of beneficial microorganisms.We used kin discrimination to construct synthetic communities(SCs)comprising 13 Bacillus subtilis strains from the surface and gut of black soldier fly(BSF)larvae.We assessed larval growth promotion in a pigeon manure system and found that the synthetic community comprising 4 strains(SC 4)had the most profound effect.Genomic analyses of these 4 strains revealed that their comple-mentary functional genes underpinned the robust functionality of the cooperative synthetic community,highlighting the importance of strain diversity.After analyzing the bacterial composition of BSF larvae and the pigeon manure substrate,we observed that SC 4 al-tered the bacterial abundance in both the larval gut and pigeon manure.This also influ-enced microbial metabolic functions and co-occurrence network complexity.Kin discrim-ination facilitates the rapid construction of synthetic communities.The positive effects of SC 4 on larval weight gain resulted from the functional redundancy and complementarity among the strains.Furthermore,SC 4 may enhance larval growth by inducing shifts in the bacterial composition of the larval gut and pigeon manure.This elucidated how the SC promoted larval growth by regulating bacterial composition and provided theoretical guidance for the construction of SCs.

community constructiongenomic analysisgut bacteriapigeon manure

Jun-Hui Zhao、Ping Cheng、Yi Wang、Xun Yan、Zhi-Min Xu、Dong-Hai Peng、Guo-Hui Yu、Ming-Wei Shao

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Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseases and Pests,Innovative Institute for Plant Health,College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,China

State Key Labora-tory of Agricultural Microbiology,National Engineering Research Center of Microbial Pesticides,College of Life Science and T

College of Resources and Environ-ment,Zhongkai University of Agriculture and Engineering,Guangzhou,China

State Key Labora-tory of Agricultural Microbiology,National Engineering Research Center of Microbial Pesticides,College of Life Science and Technology,Huazhong Agricultural University,Wuhan,China

Hubei Hongshan Laboratory,Wuhan,China

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2024

10.1111/1744-7917.13356

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(6)