首页|Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China

Genetic variation in the triosephosphate isomerase gene of the fall armyworm and its distribution across China

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The fall armyworm(FAW),Spodoptera frugiperda,has colonized and caused consistent damage in the Eastern hemisphere.The identification of various FAW strains is essential for developing precise prevention and control measures.The triosephosphate isomerase(Tpi)gene is recognized as an effective marker closely linked to FAW subpopu-lations.However,most current studies primarily focus on the comparison of variations in specific gene sites of this gene.In this study,we conducted full-length sequencing of the Tpi genes from 5 representative FAW groups.Our findings revealed that the Tpi genes var-ied in length from 1220 to 1420 bp,with the primary variation occurring within 4 introns.Notably,the exon lengths remained consistent,at 747 bp,with 37 observed base varia-tions;however,no amino acid variations were detected.Through sequence alignment,we identified 8 stable variation sites that can be used to distinguish FAW strains in the East-ern hemisphere.Additionally,we performed strain identification on 1569 FAW samples collected from 19 provinces in China between 2020 and 2021.The extensive analysis indi-cated the absence of the rice strain in the samples.Instead,we only detected the presence of the corn strain and the Zambia strain,with the Zambia strain being distributed in a very low proportion(3.44％).Furthermore,the corn strain could be further categorized into 2 subgroups.This comprehensive study provides a valuable reference for enhancing our understanding of FAW population differentiation and for improving monitoring and early warning efforts.

corn strainmolecular markerrice strainSpodoptera frugiperdaTpiZam-bia strain

Xin-Yue Liang、Lei Zhang、Hong-Ran Li、Xiao-Ping Niu、Yu-Tao Xiao

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Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Key Laboratory of Gene Editing Technologies(Hainan),Min-istry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong,China

College of Life Sciences,South China Agricultural University,Guangzhou,China

Xingping Plant Protection and Plant Quarantine Station,Xian,China

2024

10.1111/1744-7917.13348

中国昆虫科学(英文版)
中国昆虫学会 中科院动物所

中国昆虫科学(英文版)

CSTPCD
影响因子：0.484
ISSN：1672-9609
年,卷(期)：2024.31(6)