aGVHD对小鼠肠道血管形态与结构的影响及其机制
The effects and mechanism of aGVHD on the morphology and structure of murine intestinal blood vessels
郭书欣 1黄玉辉1
作者信息
- 1. 苏州大学唐仲英血液学研究中心,江苏苏州 215000
- 折叠
摘要
为了探究急性移植物抗宿主病(acute graft-versus-host disease,aGVHD)发病进程对小鼠肠道组织血管的变化和肠道免疫稳态之间的关系和机制,我们建立小鼠aGVHD模型分析小鼠小肠绒毛血管内皮细胞和周细胞以及浸润免疫细胞在不同时间点的动态变化,并运用qPCR、Smart-seq技术分析寻找差异表达基因.发现在aGVHD的发病进程中,小肠绒毛血管的损伤及浸润免疫细胞的变化与疾病进程密切相关,随炎症反应、细胞凋亡及肠道屏障相关基因表达显著变化.研究结果表明,aGVHD诱发的小肠绒毛血管的严重损伤和结构紊乱可能是导致受体死亡的一个重要原因,小肠绒毛的aGVHD炎症反应可能主要通过影响与内皮细胞凋亡及肠道屏障相关的基因表达,从而破坏小肠绒毛血管的完整性和提高其通透性.
Abstract
To explore the relationship and mechanism between the changes of intestinal tissue blood vessels and intestinal immune homeostasis in mice during the course of acute graft-versus-host disease(aGVHD),we established a mouse aGVHD model,and analyzed the dynamic changes of endothelial cells,pericyte and infiltrating immune cells in mouse small intestinal villi at different time points.qPCR and Smart-seq were used to search for differentially expressed genes.It was found that in the course of aGVHD,the damage of small intestinal villus vessels and the changes of infiltrating immune cells were closely related to the course of the disease,and significantly changed with the expression of inflammatory response,apoptosis and intestinal barrier related genes.We believe that the severe damage and structural disorder of villi vessels induced by aGVHD may be an important cause of receptor death,and the aGVHD inflammatory response of villi may damage the integrity of villi vessels and improve their permeability mainly by affecting the gene expression related to endothelial cell apoptosis and intestinal barrier.
关键词
生物学其他学科/急性移植物抗宿主病/炎症反应/肠道血管/内皮细胞/周细胞Key words
other subjects of biology/acute graft-versus-host disease/inflammatory response/intestinal vessels/endothelial cells/pericytes引用本文复制引用
出版年
2024
NETL
NSTL
万方数据