The effects and mechanism of aGVHD on the morphology and structure of murine intestinal blood vessels
To explore the relationship and mechanism between the changes of intestinal tissue blood vessels and intestinal immune homeostasis in mice during the course of acute graft-versus-host disease(aGVHD),we established a mouse aGVHD model,and analyzed the dynamic changes of endothelial cells,pericyte and infiltrating immune cells in mouse small intestinal villi at different time points.qPCR and Smart-seq were used to search for differentially expressed genes.It was found that in the course of aGVHD,the damage of small intestinal villus vessels and the changes of infiltrating immune cells were closely related to the course of the disease,and significantly changed with the expression of inflammatory response,apoptosis and intestinal barrier related genes.We believe that the severe damage and structural disorder of villi vessels induced by aGVHD may be an important cause of receptor death,and the aGVHD inflammatory response of villi may damage the integrity of villi vessels and improve their permeability mainly by affecting the gene expression related to endothelial cell apoptosis and intestinal barrier.
other subjects of biologyacute graft-versus-host diseaseinflammatory responseintestinal vesselsendothelial cellspericytes
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