Genome-wide identification and expression analysis of miR166 gene family in wheat
As an essential post-transcriptional regulator,miR166 played an important role in plant growth and development and response to stress.The evolutionary characteristics and expression patterns of miR166 gene family in wheat(Tae-miR166)were analyzed by PmiREN,Ensembl Plants database,MEGA-X,DNAMAN software,RNAfold web server,WebLogo and psRNATarget online websites.Nineteen members of the Tae-miR166 gene family were searched in the PmiREN database,and chromosome localization revealed that Tae-miR166 were localized on 14 chromosomes.Sequence alignment revealed that the mature sequences of Tae-miR166 were highly conserved;evolutionary analysis showed that the Tae-miR166 gene family members were in 4 evolutionary branches,respectively.The target gene prediction results showed that the target genes of Tae-miR166 gene family included class Ⅲ homologous heterotypic structural domain leucine zipper protein,β-galactosidase and calcium-dependent protein kinase.RNA-seq data analysis showed that 19 members of the Tae-miR166 family were expressed in 6 tissues of wheat,with the highest expression in grains and ears.The results of RT-qPCR showed that the expression patterns of Tae-miR166 family members were different after cadmium,drought and low temperature stress treatment,indicating that Tae-miR166 gene family played an important role in plant resistance to abiotic stress.This study provided a theoretical basis for further elucidating the function of miR166 gene family in wheat.
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