小麦miR166基因家族鉴定及表达分析
Genome-wide identification and expression analysis of miR166 gene family in wheat
雷晓彤 1徐渴 1孙若希 1张树华 1杨学举 1赵勇1
作者信息
- 1. 河北农业大学农学院,华北作物改良与调控国家重点实验室,华北作物种质资源研究与利用教育部重点实验室,河北保定 071001
- 折叠
摘要
miR166 作为重要的转录后调节因子,在植物的生长发育和对逆境胁迫的反应中扮演着重要的角色.利用PmiREN、Ensembl Plants数据库、MEGA-X、DNAMAN软件以及RNAfold web server、WebLogo和psRNA Target 在线网站对小麦 miR166(Tae-miR166)基因家族的进化特性和表达模式进行分析.在 PmiREN 数据库中搜索到 19 个Tae-miR166 基因家族成员,染色体定位发现Tae-miR166 成员定位于 14 条染色体上.序列比对发现Tae-miR166 的成熟体序列高度保守.进化分析结果表明,Tae-miR166 基因家族成员分别处于 4 个进化分支.靶基因预测结果表明,Tae-miR166 的靶基因包括Ⅲ类同源异型结构域亮氨酸拉链蛋白、β半乳糖苷酶和钙依赖性蛋白激酶等.转录组数据分析表明Tae-miR166 家族 19 个成员在小麦 6 个组织中都有表达,在籽粒和穗中表达量最高.荧光定量 PCR 结果表明,Tae-miR166 家族 15 个成员在镉、干旱和低温胁迫处理后的表达模式存在差异,说明 Tae-miR166 基因家族在植株抵御非生物胁迫时发挥着重要作用.本研究为进一步阐明Tae-miR166 基因家族的功能提供了理论依据.
Abstract
As an essential post-transcriptional regulator,miR166 played an important role in plant growth and development and response to stress.The evolutionary characteristics and expression patterns of miR166 gene family in wheat(Tae-miR166)were analyzed by PmiREN,Ensembl Plants database,MEGA-X,DNAMAN software,RNAfold web server,WebLogo and psRNATarget online websites.Nineteen members of the Tae-miR166 gene family were searched in the PmiREN database,and chromosome localization revealed that Tae-miR166 were localized on 14 chromosomes.Sequence alignment revealed that the mature sequences of Tae-miR166 were highly conserved;evolutionary analysis showed that the Tae-miR166 gene family members were in 4 evolutionary branches,respectively.The target gene prediction results showed that the target genes of Tae-miR166 gene family included class Ⅲ homologous heterotypic structural domain leucine zipper protein,β-galactosidase and calcium-dependent protein kinase.RNA-seq data analysis showed that 19 members of the Tae-miR166 family were expressed in 6 tissues of wheat,with the highest expression in grains and ears.The results of RT-qPCR showed that the expression patterns of Tae-miR166 family members were different after cadmium,drought and low temperature stress treatment,indicating that Tae-miR166 gene family played an important role in plant resistance to abiotic stress.This study provided a theoretical basis for further elucidating the function of miR166 gene family in wheat.
关键词
农艺学/Tae-miR166/小麦/进化特征/靶基因Key words
agronomy/Tae-miR166/wheat/evolutionary characteristics/target gene引用本文复制引用
基金项目
河北省自然科学基金(C2021204066)
出版年
2024
NETL
NSTL
万方数据