Bioinformatics analysis and target gene prediction of miR164 gene family in Triticum aestivum L.
miR164 family is a conserved small RNA molecule unique to plants,which is widely participated in plant growth and development and various stress responses.To understand the evolutionary characteristics,expression pattern,and function of wheat Tae-miR164 gene family members,bioinformatics analysis of Tae-miR164 gene was performed in PmiREN database.A total of 13 family members were identified and clustered on Chr1,Chr2,and Chr6 chromosomes of wheat.Sequence alignment showed that the mature sequences of 13 members of Tae-miR164 family all contained 21 bp,and the consistency was high.Differences existed only at the 21 nt of the 5'end.The precursor sequences could form stable secondary stem-ring structure,and the mature miRNA sequences were confined in the 5'end arm.The phylogenetic tree analysis showed that Tae-miR164 members of Arabidopsis,rice,maize,brachium dipaniculata,barley,millet,alfalfa,tomato,and wheat mainly clustered into four branches.Target genes prediction indicated that the target genes corresponding to Tae-miR164 were largely members of the NAC transcription factor.The transcriptome data analysis showed that the airport Tae-miR164a/b/c/d/e/f/g/h/I/m in wheat 6 organizations have expressed,scavenged Tae-miR164j/k/l hardly express in flowers and seeds.Real-time quantitative PCR results showed that Tae-miR164 family members showed significantly up-regulated expression pattern in the stem base of wheat treated at low temperature(4℃)for 48 h.The results of this study laid a theoretical foundation for the functional identification of the Tae-miR164 gene family in wheat.
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万方数据
