Isonicotinic acid octylidene-hydrazideassay by HPLC
Objective:Isoniazid and its derivatives are the first-line drugs for the treatment of tuberculosis,and their content determination is crucial in the development and application of drugs.However,although methods for determining the content of isoniazid are reported,there are few reports on methods for determining the content of isoniazid derivatives.Therefore,in this study,a reversed-phase high performance liquid chromatography(RP-HPLC)method suitable for the determination of isonicotinic acid octanoyl hydrazide(INH-CHO)was constructed for the content detection of INH-CHO.Methods:Capcell Pak C18 MG Ⅱ S5 chromatographic column was used,the column temperature was 30℃,the mobile phase was pH=6.0 phosphate buffer:methanol=30:70 for elution,the flow rate was 1 mL/min,and the injection volume was 0.02 mL,the detecting wavelength was 262 nm.Through the above method,the linear relationship,precision,accuracy and limit of quantification of the methodology were investigated.Results:The retention time of INH-CHO was 9.2 min,and there was a good linear relationship in the concentration range of 1-240 μg/mL and 0.1-1 μg/mL.The intra-day precision,inter-day precision and accuracy were satisfied,and the limit of quantification was 0.1 μg/mL.Conclusion:In the experimental concentration range,the method had good linearity,simple operation,accurate and reproducible results,and was suitable for the determination of INH-CHO content.
pharmaceuticsreverse high performance liquid chromatographyINH-CHOcontent quantification
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