Modified Bacillus subtilis chassis cells for efficient production of acetoin
Starting from the metabolically engineered Bacillus subtilis strain QRC-1 preserved in the early stages of laboratory cultivation,its average yield of acetoin was verified to reach 50.7 g/L in a 5 L fermentation vessel.However,issues such as self-dissolution and slow growth rate limit the biomass of Bacillus subtilis,while the foaming characteristics during fermentation affect real-time control in high-density liquid cultivation.Therefore,this experiment is based on the physiological characteristics to optimize and modify the B.subtilis parental cells,using molecular genetic techniques to develop a new strategy aimed at preventing cell lysis,enhancing biomass,and increasing acetoin production.Ultimately,the obtained strain,RKCR-8,exhibited a 30%increase in growth rate compared to the original strain,with biomass increased by 1.36 times,and acetoin yield reaching 72.3 g/L.The recombinant B.subtilis constructed in this study has stronger production efficiency and industrial stability in producing acetoin.This result not only provides more reference value for the production of acetoin but also provides a new approach for the construction of future industrial microbial chassis.
microbiologyefficient production of acetoinmolecular genetic manipulationBacillus subtilishigh-density fermentationchassis cells
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