In this paper, the species and biosynthetic process of cytokinins are introduced. Isopentenyl-transferases catalyze the rate-limiting step of cytokinin biosynthesis, and its coding genes have been cloned and used widely in plant genetic engineering .The overview of the ipt gene transformation can be increased endogenous cytokinin content which has significant effect in retard leaf senescence, increase crop yield, induced parthenocarpy, drought resistance, disease resistance and insect resistance, cold resistance and other aspects.At the same time, the paper analyzed the application and research direction of the ipt gene in plant gene engineering.