Isoflurane Inhibits Glucose Uptake in Astrocytes in Vitro
To investigate the effects of isoflurane on glucose uptake in astrocytes.Cultured rat astrocytes were exposed with isoflurane.Using immunofluorescence to examine the effect of isoflurane on astrocyte structure.Using 6-NBDG to detect the glucose absorption;Using RT-PCR and WB to detect the glucose transporters.Isoflurane induced the aggregation of GFAP to the cell membrane.Isoflurane inhibited glucose uptake,GLuT1 expression in astrocytes.Isoflurane may inhibit glucose transporters expression in astrocytes,resulting in impaired glucose uptake.