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Protein crystallization with DNA templates

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Protein crystallization plays a significant role in three-dimensional structural analysis and protein pu-rification.It is important to increase the crystallization efficiency,which is possible by adding hetero-geneous templates in crystallization systems.DNA is biologically compatible and artificially designable polymer,which is easy to extract.In this study,single-and double-stranded DNA of precise sequences were designed and used as templates to promote protein crystallization of lysozyme and catalase.In-fluence of DNA,single-stranded DNA with 10,20,40 bases and double-stranded DNA with 10,20,40 base pairs,were investigated.The success rate of obtaining crystals of lysozyme and catalase in equal period was significantly improved with the addition of DNA comparing without templates added.Double-stranded DNA led to higher nucleation rate than that with single-stranded DNA.The promotion of nucleation was more obvious at low concentration of protein solution and with longer chain DNA templates.Crystal number and crystallization rate was enhanced with addition of long double-stranded DNA templates.All the results confirm that DNA is an effective polymer additive to enhance protein crystallization,especially for the application of the scarce protein crystallization.

DNAPolymerAdditiveProteinCrystallization

Bo Zhang、Jia Yu、Zhe Pu、Wenhui Guo、Ruoqi Yang、Yu Wen、Zhijiang Rong、Hui Wang、Xiaoli Wu、Bo Wang、Hongyan Sun

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School of Environment and Resources,Taiyuan University of Science and Technology,Taiyuan,030024,China

School of Chemical Engineering and Technology,Taiyuan University of Science and Technology,Taiyuan,030024,China

PhD Scientific Research Startup Foundation of Taiyuan University of Science and TechnologyPhD Scientific Research Startup Foundation of Taiyuan University of Science and TechnologyScience and Technology Innovation Project of Shanxi Higher EducationNatural Science Foundation of Shanxi ProvinceNatural Science Foundation of Shanxi ProvinceKey Research and Development Project of Shanxi Province

20192046201920512020L036420220302121231020210302124513201903D221066

2024

10.1016/j.partic.2023.07.001

颗粒学报(英文版)
中国颗粒学会　中国科学院过程工程研究所

颗粒学报(英文版)

CSTPCD
影响因子：0.632
ISSN：1674-2001
年,卷(期)：2024.86(3)
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