首页|Optimization on crystallization process of the magnetic core lysozyme
Optimization on crystallization process of the magnetic core lysozyme
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The continuous growth of the biomedical market highlights the significance of improvement in the ef-ficiency of batch production for protein drug crystals.Herein,we investigated the effect of polymer-modified Fe3O4 nanoparticles on lysozyme(Lys)crystallization at different conditions,using function-alized Fe3O4 magnetic nanoparticles(Polymer C@Fe3O4)as nucleation agents.The study revealed that the surface charge density of Polymer C@Fe3O4 was highest at a modification mass ratio of Fe3O4 to Polymer C is 8∶3.This ratio facilitates the augmentation of electrostatic interaction and the promotion of crys-tallization.By adding 15％of the PolymerC@Fe3O4 prepared from Fe3O4 with an average particle size of 150 nm,the magnetic core lysozyme(M-Lys)crystals with an average particle size of 11.08 μm,narrow size distribution,and regular morphology were obtained in a yield of 82.42％within 60 min.
ProteinCrystallizationMagnetic particleLysozyme
Lixia Hou、Chunxiao Qu、Min Su、Zhihan Liang、Qi Hao
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School of Chemical Engineering,Hebei University of Technology,Tianjin,300130,China
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