首页|Glutamate oxidase-integrated biomimetic Fe3O4 hybrids as cascade nanozymes for glutamate detection
Glutamate oxidase-integrated biomimetic Fe3O4 hybrids as cascade nanozymes for glutamate detection
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Herein,a new nanozyme/natural enzyme hybrid biosensor was established for ultrasensitive L-glutamic acid(L-Glu)detection.The Fe3O4 nanoparticles with peroxidase-like activity and stability was used as a nanozyme and carrier for immobilizing L-Glutamate oxidase(GLOD)through Schiffff base reaction to construct a chem-enzyme cascade detector.The resultant Fe3O4-GOLD exhibited a wide linear range(10-500 μM)and a low detection limit of 6.03 μM for L-Glu detection.Furthermore,the Fe3O4-GOLD exhibited excellent pH stability,thermal stability,reusability and storage stability.After repeated nine cycles,Fe3O4-GOLD still retained 70％of its initial activity.Meanwhile,Fe3O4-GOLD maintained 50％of its initial activity after storage for 20 days,while free GLOD only retained 20％of its initial activity.This strategy of integrating biomimetic Fe3O4 and natural enzymes for cascade catalysis makes it possible to design an efficient and stable chemo-enzyme composite catalysts,which are promising for applications in biosensing and biomimetic catalysis.
State Key Laboratory of Food Nutrition and Safety,Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin,300457,China
Sports Department,Hebei University of Science and Technology,Shijiazhang,050000,China
Research Center for Fermentation Engineering of Hebei,College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhang,050000,China
Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and TechnologyScience and Technology Program of Tianjin,China
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