首页|Cross-linked α-amylase aggregates on Fe3O4 magnetic nanoparticles modified with polydopamine/polyethyleneimine for efficient hydrolysis of starch

Cross-linked α-amylase aggregates on Fe3O4 magnetic nanoparticles modified with polydopamine/polyethyleneimine for efficient hydrolysis of starch

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In this work α-amylase was immobilized on magnetic Fe3O4 nanoparticles with polyethylenimine(PEI)/polydopamine(PDA)coating or 3-aminopropyl triethoxysilane(APTES)for the first time via adsorption-precipitation-cross-linking.Compared with the free α-amylase,the resultant magnetic cross-linked α-amylase aggregates(PEI/PDA-M-CLEAs and N-M-CLEAs)exhibited excellent thermal and storage stability as well as pH stability.After storage at 25 ℃ for 60 days,free α-amylase only retained 60％of its initial activity,while PEI/PDA-M-CLEAs and N-M-CLEAs retained 80％and 78％of their initial activities,respectively.Furthermore,N-M-CLEAs and PEI/PDA-M-CLEAs showed good reusability.After 6 repeated uses,PEI/PDA-M-CLEAs and N-M-CLEAs still maintained 65％and 62％of their initial activities,respec-tively.Especially,PEI/PDA-M-CLEAs and N-M-CLEAs exhibited higher starch hydrolysis efficiency than free α-amylase.The maximum dextrose equivalent(DE)values of starch hydrolysis by PEI/PDA-M-CLEAs and N-M-CLEAs reached 29.24％and 28.79％within 90 min,respectively.However,the maximum DE values of starch hydrolysis by the free α-amylase was only 27.89％even in 150 min.The magnetic cross-linked α-amylase aggregates could be introduced as effective biocatalyst for industrial applications in production of maltose syrups.

α-amylaseMagnetic nanoparticlesFunctional modificationMagnetic cross-linked enzyme aggregatesHydrolysis of starch

Jiandong Cui、Xiuming Tang、Qingqing Ma、Yuyan Chang、Qunli Zhang、Shiru Jia

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State Key Laboratory of Food Nutrition and Safety,Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China

Key Laboratory of Biomass Chemical Engineering of Ministry of Education,Zhejiang University,Hangzhou 310027,China

Fundamental Research Funds for the Central UniversitiesScience and Technology Program of Tianjin,ChinaInternational Collaboration Project

226-2023-008520ZYJDJC000802020/37/K/ST8/03805

2024

10.1016/j.partic.2023.11.020

颗粒学报(英文版)
中国颗粒学会　中国科学院过程工程研究所

颗粒学报(英文版)

CSTPCD
影响因子：0.632
ISSN：1674-2001
年,卷(期)：2024.90(7)