首页|Surfactant-assisted crystallization of lysozyme and its mechanism
Surfactant-assisted crystallization of lysozyme and its mechanism
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Surfactants were used to assist in the crystallization of lysozyme.Three types of surfactants with different structures(sodium dodecyl sulfate,cetyltrimethylammonium bromide,or betaine hydrochlo-ride[BH])were used.The study indicated that three surfactants were all capable of shortening the crystallization induction time of lysozyme,with BH inducing the shortest crystallization induction time(t=1 min)and the largest crystal size(37 μm).The molecular interactions among each of the three surfactants aforementioned and lysozyme were also investigated by simulations.It was found that hydrogen bonding dominantly acted between the carboxyl structure of the surfactant BH and the lysozyme,which produced the most favorable conformation for the self-assembly of lysozyme(promote lysozyme orderly aggregation and shorten the crystallization induction time).On this basis,the mech-anism of surfactant-promoted lysozyme crystallization was proposed.
NETL
NSTL
万方数据
