Permeable and robust polymer-silica hybrid armor on cell catalyst for sustainable biomanufacturing

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外文摘要：Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm.However,the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell catalysis.Constructing armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the permeability.Herein,polymer-silica hybrid armor(PSHA)is directly generated on the surface of enzyme-containing cells.The branched structure of PEI enables higher porosity of cell@PSHA,exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor(SA).The electrostatic interactions(NH3+with O-)and hydrogen bonding(N…H or O…H)interactions between structural units enables higher stability of cell@PSHA,showing 3.13-fold elevation in Young's modulus compared with cell@SA.As a result,the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h,with an average yield of 77.76 g L-1.

外文关键词：

BiomanufacturingWhole cell catalysisPolymer-silica hybrid armorTagatose productionStarch conversion

作者：

Yiran Cheng、Zhenhua Wu、Boyu Zhang、Jiaxu Zhang、Jiafu Shi、Zhongyi Jiang

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作者单位：

School of Environmental Science and Engineering,Tianjin University,Tianjin,300072,China

Key Laboratory for Green Chemical Technology of Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,China

基金：

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaTianjin Synthetic Biotechnology Innovation Capacity Improvement ProjectOpen Funding Project of the State Key Laboratory of Biochemical Engineering,ChinaHaihe Laboratory of Sustainable Chemical Transformations,and Program of Introducing Talents of Discipline to Universities

项目编号：

2021YFC210230022122809TSB1C1P-KJGG-0032020 KF-06BP0618007

出版年：

2024

DOI：

10.1016/j.partic.2024.05.002

颗粒学报(英文版)

中国颗粒学会　中国科学院过程工程研究所

颗粒学报(英文版)

CSTPCD

影响因子：0.632

ISSN：1674-2001

年,卷(期)：2024.92(9)