Engineering W/O/W pickering emulsion for the enhanced antigen delivery and immune responses

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外文摘要：Tumor immunotherapy,particularly cancer vaccines,holds promise for combating cancer by harnessing tailored immune responses against malignant cells.However,conventional approaches face challenges in efficiently delivering antigens for optimal immune activation.Emulsion adjuvants,like MF59,enhance cellular uptake but struggle to induce robust CD8+T cell responses.Here,we introduce a novel strategy employing a water-in-oil-in-water(W/O/W)multiple Pickering emulsion(mPE)for antigen delivery.The mPE,utilizing biocompatible,pH-sensitive particles,encapsulates antigens within the inner water phase,ensuring enhanced intracellular processing and dictating the intracellular fate of antigens for improved cross-presentation.In vitro and in vivo studies demonstrated that mPEs induced robust dendritic cells activation and antigen cross-presentation,leading to a significantly enhanced immune response.Notably,calcium phosphate-stabilized mPE(CaP-mPE)illustrated the more robust IFN-γ+T cell responses.In comparison with traditional surfactant-stabilized multiple emulsions,CaP-mPE significantly inhibit tu-mor growth and effectively prolong the survival of tumor-bearing mice.This innovative approach offers a promising avenue for the development of effective cancer vaccines with potent cellular immune responses.

外文关键词：

Cancer vaccineMultiple pickering emulsionAdjuvantAntigen deliveryAnti-tumor response

作者：

Sihua Wu、Xiaonan Huang、Yin Ma、Naoki Asakawa、Yufei Xia

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作者单位：

State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100081,China

Key Laboratory of Biopharmaceutical Preparation and Delivery,Chinese Academy of Sciences,Beijing,100190,China

Division of Molecular Science,Graduate School of Science and Technology,Gunma University,1-5-1,Tenjin-cho,Kiryu,376-8515,Japan

Sinovac Biotech Ltd.,Beijing,100085,China

School of Chemical Engineering,Nanjing Tech University,Nanjing,211816,China

School of Chemical Engineering,University of Chinese Academy of Sciences,Beijing,100049,China

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基金：

National Key R&D Program of ChinaDistinguished Young Scholars of the National Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaMajor Program of the National Natural Science Foundation of ChinaMajor Program of the National Natural Science Foundation of ChinaKey Project of the National Natural science Foundation of ChinaCAS Project for Young Scientists in Basic ResearchFoundation for Innovative Research Groups of the National Natural Science Foundation of ChinaYouth Innovation Promotion Association of the Chinese Academy of Sciences

项目编号：

2022YFE0202500T22220222021YFC23024002021YFC2302600T2394503T239450132030062YSBR-010218210052020000053

出版年：

2024

DOI：

10.1016/j.partic.2024.05.009

颗粒学报(英文版)

中国颗粒学会　中国科学院过程工程研究所

颗粒学报(英文版)

CSTPCD

影响因子：0.632

ISSN：1674-2001

年,卷(期)：2024.92(9)