Hijacking high-flux metabolic pathways to enhance product yield in metabolic engineering
Metabolic engineering can alter metabolic processes through genetic manipulation and enzyme engineering.Metabolic engineering is widely used to produce useful compounds in pharmaceutical and chemical industries.In this context,we recategorize metabolic engineering strategies based on carbon flux.Traditional metabolic engineering strategy focuses on overexpressing or knocking out key enzymes to channel carbon flux toward the end product.However,this traditional strategy is limited when multiple bottleneck genes are involved,in which balancing gene expression often determines the efficiency of carbon flux.Here we summerize several recent research,and show the advantage of utilizing high-flux metabolic pathways as a new strategy to achieve high productivity.In addition,we also discuss the importance of fine-tuning gene expression in metabolic engineering to channel carbon flux more efficiently.
NETL
NSTL
万方数据
维普
