Post-translational modifications of protein are improtant mechanisms that regulate protein stability or biological activity in cells.The discovery of various novel post-translational modifications of proteins,especially histones,has greatly expanded the understanding of epigenetic regulation of chromatin structure and gene expression.Histones,as proteins that bind to DNA,can regulate chromatin remodeling and determine the accessibility of chromatin,and control gene expression levels throughout the entire genome.Therefore,post-translational modifications of histones are very important.The acylation modification of proteins regulated by cellular metabolism introduces acyl groups into histones,leading to changes in chromatin conformation and changes in gene expression and cell phenotypes.The technology used to study how acylation modification affects chromatin has developed rapidly and has many applications.ChIP-seq is used to study DNA sequences binding with transcription factors,and methods like DNase-seq to study the accessibility changes of chromatin.Newly emerged CUT&Tag and ATAC-seq represent the improved versions of these two experimental methods,respectively,and they are further adapted to single-cell,spatial and multi-omics experiments.Given that techniques develop very rapid,this review summarized the recent progress of commonly used methods for histone acylation,and briefly described the new methods.
NETL
NSTL
万方数据
维普
