Research status and prospect of plant complex genomes and pan-genomes
The genome refers to the sum of genetic materials within an organism and is one of the key areas of study in biology.Since the publication of the Arabidopsis genome sequence in 2000,more than 800 plant genomes have been published,which greatly promotes the development of plant molecular biology,genetics,and other fields.However,plant genomics research still faces a series of challenges,including the assembly of complex genomes with high heterozygosity,high repetitiveness,and high ploidy,as well as the construction of pan-genomes.This article comprehensively demonstrates the rapid development of plant genomics from three aspects:an overview of the development of plant genomics,genome sequencing technologies,and assembly algorithms.This review describes relevant strategies for assembling simple and complex genomes,and summarizes the assembly of"Telomere-to-Telomere"(T2T)and pan-genome construction methods,as well as their importance.Finally,the future development of plant genomics was discussed,and it was suggested that with the continuous advancement of technology,genome analysis techniques and methods will become more sophisticated,providing more support for further research on plant genomics.This review provides an important reference for research on the T2T assembly,assembly of complex genomes,and construction of pan-genomes in plants.