Viruses are a kind of microorganisms,which cause infectious diseases and pose a great threat to human health,economy,and lifestyle.Virus infection includes a series of dynamic,delicate and complex processes,involving the interaction between virus and cell components.It is difficult to accurately analyze the vital lifecycle of virus infection by traditional molecular biological methods.Single virus tracking provides a novel way for in situ and real-time visualization of the life cycle of single or multiple viruses,as well as for revealing infection mechanisms.This paper introduced the following parts:(ⅰ)virus labeling materials and methods;(ⅱ)imaging equipment and analysis platform;(ⅲ)research progress of single virus tracking.In addition,the current problems and future development trends in this field were discussed.
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