首页|RNA三维结构的测定:基于冷冻电子显微镜技术的新视角

RNA三维结构的测定:基于冷冻电子显微镜技术的新视角

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RNA在人类生物学和疾病功能障碍中发挥重要作用.对于绝大多数RNA分子而言,复杂的高级结构是其发挥功能的先决条件.了解RNA的三维结构对于研究非编码RNA功能、致病机理以及开发靶向药物至关重要.然而,由于RNA的固有灵活性,实验测定其三维结构的难度较大.冷冻电子显微镜技术(冷冻电镜,cryo-EM)得益于探测器技术和软件算法的快速发展,且不受核磁共振对小分子量及X射线晶体学对结晶的要求所制约,近年来在其他结构分析方法中脱颖而出.本文概述近年来以冷冻电子显微镜技术为核心联用其他技术(如核磁共振、晶体学和理论建模等)解析RNA三维结构的研究进展,以促进RNA结构研究和RNA靶向药物设计的发展.
Determination of RNA three-dimensional structures:a new perspective based on cryo-electron microscopy
RNAs play different functional roles in human biology and disease dysfunction.For the vast majority of RNA molecules,a complex high-level structure is a prerequisite for their function.Understanding the three-dimensional(3D)structures of non-coding RNAs is essential for studying their functions,pathogenesis,and developing targeted drugs.However,due to their inherent flexibilities,few RNA 3D structures have been determined experimentally.Cryo-electron microscopy(cryo-EM)has stood out among other structural analysis methods in recent years thanks to the rapid development of detector technology and software algorithms and is not constrained by the requirements of NMR for small molecular weights and X-ray crystallography for crystallization.In this review,we summarize the recent progress of RNA 3D structure analysis by cryo-EM in combination with other techniques(e.g.,nuclear magnetic resonance,crystallography,and theoretical modeling).The objective of this review is to facilitate a comprehensive understanding of RNA structures,enabling improved investigations into this intricate molecular composition and the development of RNA-targeted therapeutic interventions.

non-coding RNAcryo-electron microscopyRNA-targeted drugsRNA three-dimensional structuredrug design

李珊珊、安林凤、苏昭铭、张凯铭

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中国科学技术大学生命科学与医学部,合肥 230027

四川大学华西医院,生物治疗国家重点实验室,成都 610044

非编码RNA 冷冻电镜 RNA靶向药物 RNA三维结构 药物设计

国家重点研发计划国家重点研发计划中国科学院战略性先导科技专项(B类)大健康研究院前沿交叉科学与生物医学研究所项目

2022YFC23037002022YFA1302700XDB0490000QYPY20220019

2024

10.1360/SSV-2023-0196

中国科学(生命科学)
中国科学院

中国科学(生命科学)

CSTPCD北大核心
影响因子：0.725
ISSN：1674-7232
年,卷(期)：2024.54(4)
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