Long-term cryopreservation of living cells at ultralow temperatures is the key core technology to ensure the high-quality running of modem biobanks,and it has important fundamental research and clinical application value.Its standard and scale applications are playing an irreplaceable and important supporting role in clinical transfusion,drug development,plant and animal breeding and reproduction,and many other areas.Contemporary cryopreservation systems mainly focus on finding the ideal cryoprotectants,and seeking the best balance between the injuries caused by the toxicity and osmotic shocks of the cryoprotectants and their cryoprotection effects.This paper reviews the historic perspectives and progress in the cell cryopreservation field;reports authors'and peers'major achievements and technological breakthroughs in cryopreservation research and development;and envisions the future trends in cryopreservation and its great applications.
biobankcryopreservationcryoinjurysynergetic ice suppressionphysical field rewarming
赵刚、周学迅、高大勇
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中国科学技术大学电子工程与信息科学系,合肥 230027
中国医药生物技术协会组织生物样本库分会低温生物学组,上海 200093
Department of Mechanical Engineering,University of Washington,Seattle WA 98195
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