Genetically encoded calcium indicators for organelles
In eukaryotic cells,calcium(Ca2+)signal plays a vital role in regulating a wide range of physiological and pathological processes.Endoplasmic reticulum(ER),mitochondria and other Ca2+containing organelles help precisely control the spatiotemporal Ca2+dynamics and Ca2+homeostasis.Compared with chemical Ca2+indicators,the genetically encoded Ca2+indicators(GECIs)are more convenient to use,show better site-specific localization,enabling broad applications.In this review,we summarized recent advances in the development of organellar GECIs,especially those for mitochondria,ER,Golgi apparatus,endosomes and lysosome.We mainly focused on their limitations and possible future optimization strategies,aiming to provide hints to help accelerate the development of better organellar GECIs.
organelle GECIscalcium imagingcalcium signaling in subcellular compartments
万方数据
维普
