Recent advances in RNA cryo-EM structure determination
Ribonucleic acid(RNA)plays a pivotal role in the central dogma,carrying genetic information while being able to fold into complicated three-dimensional(3D)structures to regulate gene expression and to catalyze chemical reactions.The"RNA world"hypothesis proposes that RNA preceded DNA and proteins in the origin of life.However,our understanding of RNA 3D structures remains sparse compared to proteins.This perspective highlights the recent advances in RNA structure determination using single-particle cryo-electron microscopy(cryo-EM).We also compare the strengths and challenges of different structural biology methods including cryo-EM,X-ray crystallography,and nuclear magnetic resonance for determining protein-free RNA structures.We conclude by providing the prospective applications of RNA structure determination in basic science and translational research.
RNA 3D structurecryo-EM single-particle reconstructionRNA dynamics and conformational changeRNA structural designRNA-targeted molecule
万方数据
维普
