首页|治疗新冠中药中的小RNA可靶向新冠患者的差异基因

治疗新冠中药中的小RNA可靶向新冠患者的差异基因

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在中国,80％以上的2019冠状病毒病(coronavirus disease 2019,COVID-19)患者接受了传统中药(traditional Chinese medicine,TCM)治疗,其临床疗效已有报道.然而,中药治疗新冠病毒病的潜在分子机制仍不清楚.此前研究发现,中药小RNA(small RNA,sRNA)是中药新的有效成分.本研究对22名采用透解祛瘟颗粒进行治疗的新冠病毒病患者进行了队列分析.观察到服用透解祛瘟颗粒后,数千种草药小RNA进入了患者血细胞中.与此相应,患者体内下调的新冠差异表达基因与血液中检测到的高丰度中药小RNA的预测靶基因高度相关.此外,本研究计算了本草数据库中245种中药饮片所含有的丰度最高的前30条小RNA的预测靶基因占新冠病毒病患者的337个上调差异表达基因的比例,其中33种中药饮片的预测靶点所占比例超过了50％,这些中药饮片被认为是治疗COVID-19 潜在的有效饮片.其中,来自橘红、瓜蒌皮与佛手的丰度最高的前30条小RNA的预测靶基因被实验证实.本研究不仅阐明了中药对新冠病毒病患者临床疗效的新的分子机制,也为新冠治疗处方的优化提供了科学依据.
Herbal small RNAs in patients with COVID-19 linked to reduced DEG expression
In China,more than 80％of patients with coronavirus disease 2019(COVID-19)received traditional Chinese medicine(TCM)as a treatment and their clinical efficacy have been reported.However,the underlying molecular mechanism remains unclear.Previous studies have identified herbal small RNAs(sRNAs)as novel functional components.In this study,a cohort of 22 patients with COVID-19 treated with Toujie Quwen(TQ)granules was analyzed.We observed thousands of herbal small RNAs that entered the blood cells of patients after the consumption of TQ granules.In response to this treatment,the reduced differentially expressed genes(DEGs)were highly correlated with the predicted target genes of the most prevalent herbal sRNAs detected in the blood.Moreover,the predicted target genes of the top 30 sRNAs from each of the 245 TCMs in the Bencao sRNA Atlas overlapped with 337 upregulated DEGs in patients with mild COVID-19,and 33 TCMs,with more than 50％overlapping genes were identified as effective TCMs.These predicted target genes of top 30 sRNAs from Juhong,Gualoupi and Foshou were confirmed experimentally.Our results not only elucidated a novel molecular mechanism of TCM potential clinical efficacy for COVID-19 patients,but also provided 33 effective COVID-19 TCMs for prescription optimization.

traditional Chinese medicineToujie Quwen granulesCOVID-19transcriptome

乔翔宇、黄凤鸣、史晓虎、邓兴宇、张聪、梅颂、王志清、周丛照、蒋澄宇、谭行华

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中国医学科学院基础医学研究所&北京协和医学院基础学院,生物化学与分子生物学系,重大疾病共性机制研究全国重点实验室,北京 100005

中国医学科学院&北京协和医院,北京 100730

中国科学技术大学生命科学学院,合肥微尺度物质科学国家研究中心,合肥 230027

广州医科大学附属市八医院,广州 510440

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传统中药 透解祛瘟颗粒 COVID-19 转录物组测序

国家自然科学基金国家自然科学基金中国医学科学院医学与健康科技创新工程中国医学科学院捐赠经费项目高等学校学科创新引智计划2.0(111计划2.0)国家科技重大专项

81788101321001042021-I2M-1-0222021-CAMS-JZ001BP08200292018ZX10102-001-005-002

2024

10.1007/s11427-022-2225-3

中国科学(生命科学)
中国科学院

中国科学(生命科学)

CSTPCD北大核心
影响因子：0.725
ISSN：1674-7232
年,卷(期)：2024.54(8)
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