Protein-protein interaction techniques—a powerful tool for functional genomics
Cellular activities are complex and robust processes that rely on dynamic networks formed by protein-protein interactions(PPIs)for intra-and intercellular signaling and communication.The study of PPIs is,therefore,crucial for understanding protein functions and cell signaling.For the last 30 years,our team has been devoted to developing and employing various PPI techniques to systematically dissect and explore the components and mechanisms of crucial protein networks in human cells.Our primary research focuses on crucial intracellular signaling pathways,including regulatory mechanisms of telomere homeostasis,genome damage repair,and stem cell self-renewal.This review begins by elucidating commonly used PPI tools and their characteristics.Subsequently,it exemplifies how these techniques have propelled advances in biological research using key research findings from our group as an example.Finally,it outlines the potential role of artificial intelligence in advancing this field and future directions in PPI research.This comprehensive review aims to demonstrate how the development of PPI technologies promotes functional genomics research and underscores the significance of technological innovation in biology.
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