The study of glutamate reversing ofloxacin resistance in Vibrio parahaemolyticus
Bacterial resistance has become a significant public health challenge facing the international community,not only seriously endangering human health but also constraining the sustainable development of aquaculture.It is urgently needed to address the issue of antibiotic resistance.Although the understanding of bacterial resistance mechanisms continues to improve,effective control strategies are still lacking.This study focuses on the research of metabolism reversal of ofloxacin resistance in Vibrio parahaemolyticus,which seriously harms China's aquaculture industry.Vibrio parahaemolyticus ATCC17802 was selected as the experimental strain,and ofloxacin-resistant bacteria were screened by artificial passage.Using GC-MS metabolomics,the metabolic characteristics of sensitive and resistant strains of Vibrio parahaemolyticus were compared.Further analysis of the differential metabolic characteristics between sensitive and resistant strains identified 11 differential metabolic pathways,with glutamate identified as a key biomarker.Exogenous glutamate metabolism reprogramming can effectively reverse the resistance of Vibrio parahaemolyticus to ofloxacin,increasing the in vitro bactericidal rate of antibiotics by 30-fold.Moreover,this reversal effect is not only effective against Vibrio parahaemolyticus but also shows promising results against other clinically isolated antibiotic-resistant vibrio strains.Further research on the mechanism of glutamate promoting ofloxacin bactericidal action revealed that the reversal of resistance in Vibrio parahaemolyticus by glutamate is achieved through metabolic regulation mediated by increased intracellular antibiotic concentration due to proton motive force(PMF).This study expands the understanding of metabolic regulation of bacterial resistance and lays the research foundation for establishing the use of existing antibiotics to combat antibiotic-resistant infections in aquaculture.
Vibrio parahaemolyticusofloxacinglutamatemetabolic regulationproton motive force
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