Effect of the rare earth element lanthanum on community dynamics of methanol-grown fecal microbiota
Rare earth element lanthanum(La3+)is widely used in industry,agriculture and biomedicine.Previous studies have shown that La3+plays a key role in methylotrophic regulation,but little is known about its role in human gut microbiome.Here,we reported the dynamics of bacterial communities in response to La3+stimulation in human fecal samples by combining culturomics and high-throughput sequencing.Bacterial samples were cultured in strongly selective medium nitrate minimal salts(NMS)medium with methanol as the sole carbon source.Through measurements of the community-composition of enriched cultures under different treatments,we found that,Methylophilaceae family were dominant in the fecal culture samples.Furthermore,the Methylophilus was identified as the predominant genus.Additionally,the species diversity in the La3+-added group was significantly reduced and the composition of species in cultured samples altered as well.Meanwhile,the addition of La3+could promote the degree of connectivity between Methylophilus and other genera.This study not only affirms the crucial role of methylotrophic bacteria in preserving the structure and function of the intestinal microecosystem but also introduces innovative avenues for exploring methods and mechanisms that elucidate the participation of gut bacteria in single carbon metabolism of the human body.
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