首页|Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering

Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering

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The phase behaviors of the complex formed by didodecyldimethylammonium bromide (DDAB) and cetyltrimethylammonium bromide (CTAB) interacting with three different types of DNAs,salmon testes DNA (～2000 bp),21-bp double-stranded oligonucleotides (oligo-dsDNA),and 21-nt single-stranded oligonucleotides (oligo-ssDNA) were studied by synchrotron small-angle X-ray scattering.It was found that the DNA length and flexibility,together with the positive/negative charge ratio,determined the final structure.At higher charge ratios,the DNA length exhibited negligible effect.Both oligo-dsDNA and salmon DNA formed inverted hexagonal packing of cylinders with CTAB,as well as bilayered lamella with DDAB.However,at lower charge ratios,oligo-dsDNA formed a distorted hexagonal phase with CTAB and a new structure with DDAB,which was different from the behaviors of salmon DNA.The flexible oligo-ssDNA formed rich structures that were subject to environmental disturbance.Kinetic study also indicated that the structures of the complex formed by oligo-ssDNA took much longer to mature than the structures formed by oligo-dsDNA.We attributed this result to the conformational adjustment of oligo-ssDNA in the complex.

phase behaviorDNAsurfactantsynchrotron small-angle X-ray scatteringkinetics

NIU Lin、YAN JingJing、YANG XuYan、BURGER Christian、RONG LiXia、HSIAO Benjamin、LIANG DeHai

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Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education

Department of Polymer Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794, USA

This work was financially supported by the National Natural Science Foundation of ChinaNational Basic Research Program of China

211740072012CB821500

2014

10.1007/s11426-014-5159-y

中国科学:化学(英文版)
中国科学院

中国科学:化学(英文版)

CSCDSCIEI
影响因子：0.629
ISSN：1674-7291
年,卷(期)：2014.57(12)
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