首页|KLF4 facilitates chromatin accessibility remodeling in porcine early embryos

KLF4 facilitates chromatin accessibility remodeling in porcine early embryos

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Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illu-strated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 over-expression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo devel-opment.

porcine embryosliDNase-seqchromatin accessibilityKLF4mitochondrionlipid droplets

Wei Zhu、Guowei Bu、Ruifeng Hu、Jixiang Zhang、Lianyong Qiao、Kai Zhou、Tingting Wang、Qiao Li、Jingjing Zhang、Linhui Wu、Yali Xie、Taotao Hu、Shichun Yang、Jiaqi Guan、Xiaoyu Chu、Juanjuan Shi、Xia Zhang、Falong Lu、Xin Liu、Yi-Liang Miao

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Institute of Stem Cell and Regenerative Biology,College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China

Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction(Huazhong Agricultural University),Ministry of Education,Wuhan 430070,China

State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China

Frontiers Science Center for Animal Breeding and Sustainable Production,Huazhong Agricultural University,Wuhan 430070,China

Hubei Hongshan Laboratory,Wuhan 430070,China

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National Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaStrategic Priority Research Program of Chinese Academy of SciencesKey Research and Development Program of Hubei ProvinceMajor Project of Hubei Hongshan LaboratoryFoundation of Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Educatio

319021612022YFD13022012018YFA0107001XDA240202032021BBA2212021hszd003QJHKY[2022]373

2024

10.1007/s11427-022-2349-9

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(1)
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