首页|Identification and characterization of human hematopoietic mesoderm

Identification and characterization of human hematopoietic mesoderm

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The embryonic mesoderm comprises heterogeneous cell subpopulations with distinct lineage biases.It is unclear whether a bias for the human hematopoietic lineage emerges at this early developmental stage.In this study,we integrated single-cell transcriptomic analyses of human mesoderm cells from embryonic stem cells and embryos,enabling us to identify and define the molecular features of human hematopoietic mesoderm(HM)cells biased towards hematopoietic lineages.We discovered that BMP4 plays an essential role in HM specification and can serve as a marker for HM cells.Mechanistically,BMP4 acts as a downstream target of HDAC1,which modulates the expression of BMP4 by deacetylating its enhancer.Inhibition of HDAC significantly enhances HM specification and promotes subsequent hematopoietic cell differentiation.In conclusion,our study identifies human HM cells and describes new mechanisms for human hema-topoietic development.

hematopoietic mesodermhuman pluripotent stem cellshuman embryosBMP4HDAC1

Yuqi Wen、Jingjing Zhao、Runqing Zhang、Fan Liu、Xiaoyuan Chen、Dan Wu、Mengge Wang、Cuicui Liu、Pei Su、Panpan Meng、Yiyue Zhang、Xin Gao、Lu Wang、Hongtao Wang、Jiaxi Zhou

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State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Haihe Laboratory of Cell Ecosystem,Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Tianjin 300020,China

Tianjin Institutes of Health Science,Tianjin 301600,China

CAMS Center for Stem Cell Medicine,PUMC Department of Stem Cell and Regenerative Medicine,Tianjin 300020,China

Division of Cell,Developmental and Integrative Biology,School of Medicine,South China University of Technology,Guangzhou 510006,China

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CAMS Innovation Fund for Medical SciencesCAMS Innovation Fund for Medical SciencesNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaHaihe Laboratory of Cell Ecosystem Innovation FundNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaTianjin Municipal Science and Technology Commission GrantTianjin Municipal Science and Technology Commission GrantTianjin Municipal Science and Technology Commission Grant

2021-I2M-1-073.2021-I2M-1-0402022-I2M-JB-0152021YFA11007032021YFA110300022HHXBSS0003182125003322711618220014120JCYBJC0024022ZXSYSY0001022JCQNJC01270

2024

10.1007/s11427-022-2374-x

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(2)
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