The light and hypoxia induced gene ZmPORB1 determines tocopherol content in the maize kernel

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外文摘要：Tocopherol is an important lipid-soluble antioxidant beneficial for both human health and plant growth.Here,we fine mapped a major QTL-qVE1 affecting γ-tocopherol content in maize kernel,positionally cloned and confirmed the underlying gene ZmPORB1(por1),as a proto-chlorophyllide oxidoreductase.A 13.7 kb insertion reduced the tocopherol and chlorophyll content,and the photosynthetic activity by repressing ZmPORB1 expression in embryos of NIL-K22,but did not affect the levels of the tocopherol precursors HGA(homogentisic acid)and PMP(phytyl monophosphate).Furthermore,ZmPORB1 is inducible by low oxygen and light,thereby involved in the hypoxia response in developing embryos.Concurrent with natural hypoxia in embryos,the redox state has been changed with NO increasing and H2O2 de-creasing,which lowered γ-tocopherol content via scavenging reactive nitrogen species.In conclusion,we proposed that the lower light-harvesting chlorophyll content weakened embryo photosynthesis,leading to fewer oxygen supplies and consequently diverse hypoxic responses including an elevated γ-tocopherol consumption.Our findings shed light on the mechanism for fine-tuning endogenous oxygen concentration in the maize embryo through a novel feedback pathway involving the light and low oxygen regulation of ZmPORB1 expression and chlorophyll content.

外文关键词：

maizetocopherolhypoxiaphotosynthesisZmPORB1

作者：

Nannan Liu、Yuanhao Du、Shijuan Yan、Wei Chen、Min Deng、Shutu Xu、Hong Wang、Wei Zhan、Wenjie Huang、Yan Yin、Xiaohong Yang、Qiao Zhao、Alisdair R.Fernie、Jianbing Yan

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作者单位：

National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China

Hubei Hongshan Laboratory,Wuhan 430070,China

Agro-biological Gene Research Center,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China

College of Agronomy,Hunan Agricultural University,Changsha 410128,China

College of Agronomy,Northwest A&F University,Xi'an 710000,China

State Key Laboratory of North China Crop Improvement and Regulation,Hebei Sub-center of National Maize Improvement Center of China,College of Agronomy,Hebei Agricultural University,Baoding 071001,China

College of Life Sciences,South-Central Minzu University,Wuhan 430070,China

Plant Science Facility of the Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China

National Maize Improvement Center of China,Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China

Shenzhen Key Laboratory of Synthetic Genomics,Guangdong Provincial Key Laboratory of Synthetic Genomics,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Max Planck Institute of Molecular Plant Physiology,Potsdam-Golm 14476,Germany

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基金：

国家自然科学基金国家自然科学基金国家重点研发计划Key Area Research and Development Program of Guangdong Province,ChinaHuazhong Agricultural University Scientific &Technological Self-Innovation Foundation

项目编号：

32200221U19012012022YFD12015022022B0202060003

出版年：

2024

DOI：

10.1007/s11427-023-2489-2

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(3)

参考文献量61