首页|Integrated neural tracing and in-situ barcoded sequencing reveals the logic of SCN efferent circuits in regulating circadian behaviors

Integrated neural tracing and in-situ barcoded sequencing reveals the logic of SCN efferent circuits in regulating circadian behaviors

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The circadian clock coordinates rhythms in numerous physiological processes to maintain organismal homeostasis.Since the su-prachiasmatic nucleus(SCN)is widely accepted as the circadian pacemaker,it is critical to understand the neural mechanisms by which rhythmic information is transferred from the SCN to peripheral clocks.Here,we present the first comprehensive map of SCN efferent connections and suggest a molecular logic underlying these projections.The SCN projects broadly to most major regions of the brain,rather than solely to the hypothalamus and thalamus.The efferent projections from different subtypes of SCN neurons vary in distance and intensity,and blocking synaptic transmission of these circuits affects circadian rhythms in locomotion and feeding to different extents.We also developed a barcoding system to integrate retrograde tracing with in-situ sequencing,allowing us to link circuit anatomy and spatial patterns of gene expression.Analyses using this system revealed that brain regions functioning downstream of the SCN receive input from multiple neuropeptidergic cell types within the SCN,and that individual SCN neurons generally project to a single downstream brain region.This map of SCN efferent connections provides a critical foundation for future investigations into the neural circuits underlying SCN-mediated rhythms in physiology.Further,our new barcoded tracing method provides a tool for revealing the molecular logic of neuronal circuits within heterogeneous brain regions.

circadian rhythmsSCNoutput circuitneural tracingbarcoded GFPin situ sequencing

Meimei Liao、Xinwei Gao、Chen Chen、Qi Li、Qingchun Guo、He Huang、Erquan Zhang、Dapeng Ju

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College of Biological Sciences,China Agriculture University,Beijing 100193,China

National Institute of Biological Sciences(NIBS),Beijing 102206,China

Chinese Institute for Brain Research,Beijing 102206,China

Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing 102206,China

School of Biomedical Engineering,Capital Medical University,Beijing 100069,China

Department of Anesthesiology,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 401336,China

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国家自然科学基金国家自然科学基金科技部项目Kuanren Talents'Project of The Second Affiliated Hospital of Chongqing Medical University

32171157319710902021ZD0203400

2024

10.1007/s11427-023-2420-7

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(3)
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