首页|Complicated target recognition by archaeal box C/D guide RNAs

Complicated target recognition by archaeal box C/D guide RNAs

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Box C/D RNAs guide the site-specific formation of 2'-O-methylated nucleotides(Nm)of RNAs in eukaryotes and archaea.Although C/D RNAs have been profiled in several archaea,their targets have not been experimentally determined.Here,we mapped Nm in rRNAs,tRNAs,and abundant small RNAs(sRNAs)and profiled C/D RNAs in the crenarchaeon Sulfolobus islandicus.The targets of C/D RNAs were assigned by analysis of base-pairing interactions,in vitro modification assays,and gene deletion experiments,revealing a complicated landscape of C/D RNA-target interactions.C/D RNAs widely use dual antisense elements to target adjacent sites in rRNAs,enhancing modification at weakly bound sites.Two consecutive sites can be guided with the same antisense element upstream of box D or D',a phenomenon known as double-specificity that is exclusive to internal box D'in eukaryotic C/D RNAs.Several C/D RNAs guide modification at a single non-canonical site.This study reveals the global landscape of RNA-guided 2'-O-methylation in an archaeon and unexpected targeting rules employed by C/D RNA.

C/D RNA2'-O-methylationarchaeaguide RNARNA modification

Jiayin Wang、Songlin Wu、Keqiong Ye

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Key Laboratory of RNA Science and Engineering,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

University of Chinese Academy of Sciences,Beijing 100049,China

中国科学院战略规划重点项目中国科学院战略规划重点项目Basic Research Program Based on Major Scientific Infrastructures of Chinese Academy of Sciences国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家重点研发计划

XDB0570000XDB37010201JZHKYPT-2021-05919403029154020131430024313250072017YFA0504600

2024

10.1007/s11427-022-2412-3

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(4)
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