首页|The spatial transcriptomic landscape of human gingiva in health and periodontitis

The spatial transcriptomic landscape of human gingiva in health and periodontitis

扫码查看
原文链接
  • 万方数据
  • 维普
The gingiva is a key oral barrier that protects oral tissues from various stimuli.A loss of gingival tissue homeostasis causes periodontitis,one of the most prevalent inflammatory diseases in humans.The human gingiva exists as a complex cell network comprising specialized structures.To understand the tissue-specific pathophysiology of the gingiva,we applied a recently developed spatial enhanced resolution omics-sequencing(Stereo-seq)technique to obtain a spatial transcriptome(ST)atlas of the gingiva in healthy individuals and periodontitis patients.By utilizing Stereo-seq,we identified the major cell types present in the gingiva,which included epithelial cells,fibroblasts,endothelial cells,and immune cells,as well as subgroups of epithelial cells and immune cells.We further observed that inflammation-related signalling pathways,such as the JAK-STAT and NF-κB signalling pathways,were significantly upregulated in the endothelial cells of the gingiva of periodontitis patients compared with those of healthy individuals.Additionally,we characterized the spatial distribution of periodontitis risk genes in the gingiva and found that the expression of IFI16 was significantly increased in endothelial cells of inflamed gingiva.In conclusion,our Stereo-seq findings may facilitate the development of innovative therapeutic strategies for periodontitis by mapping periodontitis-relevant genes and pathways and effector cells.

spatial transcriptomicsgingivaperiodontal diseaseperiodontitis risk genes

Zongshan Shen、Ran Zhang、Yunjia Huang、Jiayao Chen、Mengjun Yu、Chunhua Li、Yong Zhang、Lingling Chen、Xin Huang、Jichen Yang、Zhengmei Lin、Songlin Wang、Bin Cheng

展开 >

Hospital of Stomatology,Guangdong Provincial Key Laboratory of Stomatology,Guanghua School of Stomatology,Sun Yat-sen University,Guangzhou 510055,China

Beijing Laboratory of Oral Health,Capital Medical University,Beijing 100050,China

Department of Oral Pathology,Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices,Beijing 100050,China

Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions,Chinese Academy of Medical Sciences,Beijing 100050,China

BGI-Qingdao,BGI-Shenzhen,Qingdao 266555,China

展开 >

国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金Beijing Municipal Government grant(Beijing Laboratory of Oral Health)Chinese Academy of Medical Sciences Innovation Fund for Medical SciencesScience and Technology Project of Guangzhou,ChinaPostdoctoral Foundation of ChinaPostdoctoral Foundation of China

82201011820300319214930182270945PXM2021-014226-0000412019-I2M-5-0382022060800092021M7036952021TQ0308

2024

10.1007/s11427-023-2467-1

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(4)
  • 36