Dynamic changes in butyrate levels regulate satellite cell homeostasis by preventing spontaneous activation during aging

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外文摘要：The gut microbiota plays a pivotal role in systemic metabolic processes and in particular functions,such as developing and preserving the skeletal muscle system.However,the interplay between gut microbiota/metabolites and the regulation of satellite cell(SC)homeostasis,particularly during aging,remains elusive.We propose that gut microbiota and its metabolites modulate SC physiology and homeostasis throughout skeletal muscle development,regeneration,and aging process.Our investigation reveals that microbial dysbiosis manipulated by either antibiotic treatment or fecal microbiota transplantation from aged to adult mice,leads to the activation of SCs or a significant reduction in the total number.Furthermore,employing multi-omics(e.g.,RNA-seq,16S rRNA gene sequencing,and metabolomics)and bioinformatic analysis,we demonstrate that the reduced butyrate levels,alongside the gut microbial dysbiosis,could be the primary factor contributing to the reduction in the number of SCs and subsequent impairments during skeletal muscle aging.Meanwhile,butyrate supplementation can mitigate the antibiotics-induced SC activation irrespective of gut microbiota,potentially by inhibiting the proliferation and differentiation of SCs/myoblasts.The butyrate effect is likely facilitated through the monocarboxylate transporter 1(Mct1),a lactate transporter enriched on membranes of SCs and myoblasts.As a result,butyrate could serve as an alternative strategy to enhance SC homeostasis and function during skeletal muscle aging.Our findings shed light on the potential application of microbial metabolites in maintaining SC homeostasis and preventing skeletal muscle aging.

外文关键词：

gut microbiotaagingsatellite cellsbutyrateSlc16a1(Mct1)

作者：

Shujie Chen、Liujing Huang、Bingdong Liu、Huimin Duan、Ze Li、Yifan Liu、Hu Li、Xiang Fu、Jingchao Lin、Yinlan Xu、Li Liu、Dan Wan、Yulong Yin、Liwei Xie

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作者单位：

Department of Endocrinology and Metabolism,Zhujiang Hospital,Southern Medical University,Guangzhou 510280,China

State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China

Department of Rehabilitation Medicine,Nanfang Hospital,Southern Medical University,Guangzhou 510000,China

School of Public Health,Xinxiang Medical University,Xinxiang 453003,China

Institute of Aging Research,Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,School of Medical Technology,Guangdong Medical University,Dongguan 524023,China

Bioland Laboratory,Guangzhou Regenerative Medicine and Health Guangdong Laboratory,Guangzhou 510005,China

Metabo-Profile Biotechnology(Shanghai)Co.Ltd.,Shanghai 201315,China

Department of Infectious Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China

Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

Department of Infectious Diseases,Nanfang Hospital,So

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基金：

Guangdong Basic and Applied Basic Research Foundation"GDAS"Project of Science and Technology Development"GDAS"Project of Science and Technology Development国家自然科学基金国家自然科学基金Open Program of Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsOutstanding Youth Fund of Hunan Natural Science Foundation中国科学院青年创新促进会项目

项目编号：

202OB15150200462021GDASYL-202101020032018GDASCX-01028207243632130099GPKLMMD-OP2021022021JJ200452022370

出版年：

2024

DOI：

10.1007/s11427-023-2400-3

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(4)

参考文献量93