首页|Hi-Tag:a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers
Hi-Tag:a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers
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Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation.However,these techniques require complex experimental procedures and millions of cells per experiment,which limits their widespread application in life science research.Here,we develop a novel method,Hi-Tag,that identifies high-resolution,long-range chromatin inter-actions through transposase tagmentation and chromatin proximity ligation(with a phosphorothioate-modified linker).Hi-Tag can be implemented using as few as 100,000 cells,involving simple experimental procedures that can be completed within 1.5 days.Meanwhile,Hi-Tag is capable of using its own data to identify the binding sites of specific proteins,based on which,it can acquire accurate interaction information.Our results suggest that Hi-Tag has great potential for advancing chromatin interaction studies,particularly in the context of limited cell availability.
Key Lab of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China
Hubei Hongshan Laboratory,Frontiers Science Center for Animal Breeding and Sustainable Production,Wuhan 430070,China
National Natural Science Foundation of ChinaEarmarked Fund for CARSNational Natural Science Foundation of China Outstanding YouthMajor Project of Hubei Hongshan Laboratory
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