首页|Upgraded durian genome reveals the role of chromosome reshuffling during ancestral karyotype evolution,lignin bio-synthesis regulation,and stress tolerance

Upgraded durian genome reveals the role of chromosome reshuffling during ancestral karyotype evolution,lignin bio-synthesis regulation,and stress tolerance

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Durian(Durio zibethinus)is a tropical fruit that has a unique flavor and aroma.It occupies a significant phylogenetic position within the Malvaceae family,Extant core-eudicot plants are reported to share seven ancestral karyotypes that have undergone reshuffling,resulting in an abundant genomic diversity.However,the ancestral karyotypes of the Malvaceae family,as well as the evolution trajectory leading to the 28 chromosomes in durian,remain poorly understood.Here,we report the high-quality assembly of the durian genome with comprehensive comparative genomic analyses.By analyzing the collinear blocks between cacao and durian,we inferred 11 Malvaceae ancestral karyotypes.These blocks were present in a single-copy form in cacao and mainly in triplicates in durian,possibly resulting from a recent whole genome triplication(WGT)event that led to hexaploidization of the durian genome around 20(17-24)million years ago.A large proportion of the duplicated genes in durian,such as those involved in the lignin biosynthesis module for phenylpropane biosynthesis,are derived directly from whole genome duplication,which makes it an important force in reshaping its genomic architecture.Transcriptome studies have revealed that genes involved in feruloyl-CoA formations were highly preferentially expressed in fruit peels,indicating that the thorns produced on durian fruit may comprise guaiacyl and syringyl lignins.Among all the analyzed transcription factors(TFs),members of the heat shock factor family(HSF)were the most significantly upregulated under heat stress.All subfamilies of genes encoding heat shock proteins(HSPs)in the durian genome appear to have undergone expansion.The potential interactions between HSF Dzi05.397 and HSPs were examined and experimentally verified.Our study provides a high-quality durian genome and reveals the reshuffling mechanism of ancestral Malvaceae chromosomes to produce the durian genome.We also provide insights into the mechanism underlying lignin bio-synthesis and heat stress tolerance.

Durio zibethinuswhole genome duplicationwhole genome triplicationMalvaceae ancestral karyotypeschromosome reshufflinglignin biosynthesisheat stress

Wanwan Li、Xin Chen、Jianing Yu、Yuxian Zhu

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College of Life Sciences,Shaanxi Normal University,Xi'an 710119,China

The State Key Laboratory of Protein and Gene Research,College of Life Sciences,Peking University,Beijing 100871,China

The Institute for Advanced Studies,Wuhan University,Wuhan 430072,China

Shaanxi Normal University Academician Expert Workstation FoundationNational Key Laboratory of Protein and Plant Gene Research Open Project

1110010375ZD2021058

2024

10.1007/s11427-024-2580-3

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(6)
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