Advances in environmental DNA monitoring:standardization,automation,and emerging technologies in aquatic ecosystems

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外文摘要：Environmental DNA(eDNA)monitoring,a rapidly advancing technique for assessing biodiversity and ecosystem health,offers a non-invasive approach for detecting and quantifying species from various environmental samples.In this review,a comprehensive overview of current eDNA collection and detection technologies is provided,emphasizing the necessity for standardization and automation in aquatic ecological monitoring.Furthermore,the intricacies of water bodies,from streams to the deep sea,and the associated challenges they pose for eDNA capture and analysis are explored.The paper delineates three primary eDNA survey methods,namely,bringing back water,bringing back filters,and bringing back data,each with specific advantages and constraints in terms of labor,transport,and data acqui-sition.Additionally,innovations in eDNA sampling equipment,including autonomous drones,subsurface samplers,and in-situ filtration devices,and their applications in monitoring diverse taxa are discussed.Moreover,recent advancements in species-specific detection and eDNA metabarcoding are addressed,highlighting the integration of novel techniques such as CRISPR-Cas and nanopore sequencing that enable precise and rapid detection of biodiversity.The implications of environmental RNA and epigenetic modifications are considered for future applications in providing nuanced ecological data.Lastly,the review stresses the critical role of standardization and automation in enhancing data consistency and comparability for robust long-term biomonitoring.We propose that the amalgamation of these technologies represents a paradigm shift in ecological monitoring,aligning with the urgent call for biodiversity conservation and sustainable manage-ment of aquatic ecosystems.

外文关键词：

eDNAmethodologysamplerenvironmentbiomonitoringecogenomic sensorsocean observationinstrument

作者：

Suxiang Lu、Honghui Zeng、Fan Xiong、Meng Yao、Shunping He

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作者单位：

State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China

Institute of Ecology,College of Urban and Environmental Sciences,Peking University,Beijing 100871,China

School of Life Sciences,Peking University,Beijing 100871,China

Institute of Deep Sea Science and Engineering,Chinese Academy of Sciences,Sanya,China

Center for Excellence in Animal Evolution and Genetics,Chinese Academy of Sciences,Kunming,China

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基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaSpecial Project for Social Development of Yunnan Province

项目编号：

42330405322003672022YFF060820032325034U2340216202103AC100001

出版年：

2024

DOI：

10.1007/s11427-023-2493-5

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(7)