Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor

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外文摘要：Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins,showing a potential threat to humans.Here we report the infectivity and pathogenicity of the SARS-CoV-2-related virus,PCoV-GX/P2V,which was isolated from a Malayan pangolin(Manis javanica).PCoV-GX/P2V could grow in human hepatoma,colorectal adenocarcinoma cells,and human primary nasal epithelial cells.It replicated more efficiently in cells expressing human angiotensin-converting enzyme 2(hACE2)as SARS-CoV-2 did.After intranasal in-oculation to the hACE2-transgenic mice,PCoV-GX/P2V not only replicated in nasal turbinate and lungs,but also caused interstitial pneumonia,characterized by infiltration of mixed inflammatory cells and multifocal alveolar hemorrhage.Existing population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V infection.These findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission.

外文关键词：

pangolinSARS-CoV-2-related virusinfectivitypathogenicityhuman cell lineshuman ACE2 receptorhACE2-transgenic mice

作者：

Luo-Yuan Xia、Xue-Feng Wang、Xiao-Ming Cui、Yi-Ming Zhang、Zhen-Fei Wang、En-Tao Li、Chang-Fa Fan、Ke Song、Yuan-Guo Li、Run-Ze Ye、Fang-Xu Li、Dai-Yun Zhu、Jie Zhang、Zhuang-Zhuang Shi、Ming-Zhu Zhang、Liang-Jing Li、Shi-Jing Shen、Song Jin、Ya-Wei Zhang、Wei-Guang Fu、Lin Zhao、Wen-Hao Wang、Tie-Cheng Wang、You-Chun Wang、Jia-Fu Jiang、Yan-Ling Hu、Na Jia、Yu-Wei Gao、Wu-Chun Cao

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作者单位：

Institute of EcoHealth,School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China

State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China

Changchun Veterinary Research Institute,Changchun 130122,China

Research Unit of Discovery and Tracing of Natural Focus Diseases,Chinese Academy of Medical Sciences,Beijing 100071,China

College of Life Sciences,Shandong Normal University,Jinan 250014,China

Division of Animal Model Research,Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control,Beijing 102629,China

National Institutes for Food and Drug Control,Beijing 102629,China

Life Sciences Institute,Guangxi Medical University,Nanning 530020,China

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基金：

National Natural Science Foundation of China

项目编号：

81621005

出版年：

2024

DOI：

10.1007/s11427-023-2484-x

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(7)