首页|Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR

Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR

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Engineering disease-resistant plants can be a powerful solution to the issue of food security.However,it requires addressing two funda-mental questions:what genes to express and how to control their expressions.To find a solution,we screen CRISPR-edited upstream open reading frame(uORF)variants in rice,aiming to optimize translational control of disease-related genes.By switching uORF types of the 5'-leader from Arabidopsis TBF1,we modulate the ribosome accessibility to the downstream firefly luciferase.We assume that by switching uORF types using CRISPR,we could generate uORF variants with alternative translation efficiency(CRISPR-aTrE-uORF).These variants,capable of boosting translation for resistance-associated genes and dampening it for susceptible ones,can help pinpoint previously uni-dentified genes with optimal expression levels.To test the assumption,we screened edited uORF variants and found that enhanced translational suppression of the plastic glutamine synthetase 2 can provide broad-spectrum disease resistance in rice with minimal fitness costs.This strategy,which involves modifying uORFs from none to some,or from some to none or different ones,demonstrates how translational agriculture can speed up the development of disease-resistant crops.This is vital for tackling the food security challenges we face due to growing populations and changing climates.

disease-resistant plantsbroad-spectrum disease resistanceuORFuORF typesuORF variantsCRISPR

Jingjing Tian、Zhijuan Tang、Ruixia Niu、Yulu Zhou、Dan Yang、Dan Chen、Ming Luo、Rui Mou、Meng Yuan、Guoyong Xu

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National Key Laboratory of Crop Genetic Improvement,National Centre of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China

State Key Laboratory of Hybrid Rice,Institute for Advanced Studies(IAS),Wuhan University,Wuhan 430072,China

Hubei Hongshan Laboratory,Wuhan 430070,China

National Key Research and Development Program of ChinaMajor Project of Hubei Hongshan LaboratoryNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaKey Research and Development Program of Hubei ProvinceFundamental Research Funds for the Central Universities

2023ZD040732022hszd01632070284321724212022BFE0032662023PY006

2024

10.1007/s11427-024-2588-9

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(8)
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