首页|LPS-related muscle loss is associated with the alteration of Bacteroidetes abundance,systemic inflammation,and mito-chondrial morphology in a weaned piglet model
LPS-related muscle loss is associated with the alteration of Bacteroidetes abundance,systemic inflammation,and mito-chondrial morphology in a weaned piglet model
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
We previously demonstrated that lipopolysaccharide(LPS)injection-induced immune stress could impair muscle growth in weaned piglets,but the precise mechanisms behind this remain elusive.Here,we found that chronic immune stress induced by LPS resulted in a significant reduction of 36.86％in the total muscle mass of piglets at 5 d post-treatment compared with the control group.At 1 d,prior to muscle mass loss,multiple alterations were noted in response to LPS treatment.These included a reduction in the abundance of Bacteroidetes,an increase in serum concentrations of pro-inflammatory cytokines,compromised mitochondrial morphology,and an upregulation in the expression of dynamin-related protein 1(Drp1),a critical protein involved in mitochondrial fission.We highlight a strong negative correlation between Bacteroidetes abundance and the levels of serum pro-inflammatory cytokines,cor-roborated by in vivo intervention strategies in the musculature of both pig and mouse models.Mechanistically,the effects of Bac-teroidetes on inflammation and muscle mass loss may involve the signaling pathway of the tauro-β-muricholic acid-fibroblast growth factor 15.Furthermore,the induction of overexpression of inflammatory cytokines,achieved without LPS treatment through oral administration of recombinant human IL-6(rhIL-6),led to increased levels of circulating cytokines,subsequently causing a decrease in muscle mass.Notably,pre-treatment with Mdivi-1,an inhibitor of Drp-1,markedly attenuated the LPS-induced elevation in reactive oxygen species levels and rescued the associated decline in muscle mass.Collectively,these data indicate that LPS-induced muscle mass loss was linked to the reduction of Bacteroidetes abundance,increased inflammation,and the disruption of mitochondrial morphology.These insights offer promising avenues for the identification of potential therapeutic targets aimed at mitigating muscle mass loss.
Bacteroidetesinflammationmitochondrial morphologymuscle mass losspiglets
Jiayi Yu、Changbing Zheng、Qiuping Guo、Yulong Yin、Yehui Duan、Fengna Li
展开 >
CAS Key Laboratory of Agro-ecological Processes in Subtropical Region,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China
University of Chinese Academy of Sciences,Beijing 100049,China
College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China
National Natural Science Foundation of ChinaScience and Technology Innovation Program of Hunan ProvinceChangsha Natural Science Funds for Distinguished Young ScholarNational Key Research and Development Programs of ChinaChina Agriculture Research System of MOF and MARA
万方数据
