首页|The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors

The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors

扫码查看
原文链接
  • 万方数据
Mammalian hair cells(HCs)are arranged spirally along the cochlear axis and correspond to different frequency ranges.Serving as primary sound detectors,HCs spatially segregate component frequencies into a topographical map.HCs display significant diversity in anatomical and physiological characteristics,yet little is known about the organization of the cochleotopic map of HCs or the molecules involved in this process.Using single-cell RNA sequencing,we determined the distinct molecular profiles of inner hair cells and outer hair cells,and we identified numerous position-dependent genes that were expressed as gradients.Newly identified genes such as Ptn,Rxra,and Nfe212 were found to be associated with tonotopy.We employed the SCENIC algorithm to predict the transcription factors that potentially shape these tonotopic gradients.Furthermore,we confirmed that Nfe212,a tonotopy-related transcription factor,is critical in mice for sensing low-to-medium sound frequencies in vivo.the analysis of cell-cell communication revealed potential receptor-ligand networks linking inner hair cells to spiral ganglion neurons,including pathways such as BDNF-Ntrk and PTN-Scd4,which likely play essential roles in tonotopic maintenance.Overall,these findings suggest that molecular gradients serve as the organizing principle for maintaining the selection of sound frequencies by HCs.

tonotopic axishair cellauditory receptorscRNA-seq

Xiangyu Ma、Xin Chen、Yuwei Che、Siyao Zhu、Xinlin Wang、Shan Gao、Jiheng Wu、Fanliang Kong、Cheng Cheng、Yunhao Wu、Jiamin Guo、Jieyu Qi、Renjie Chai

展开 >

State Key Laboratory of Bioelectronics,Department of Otolaryngology Head and Neck Surgery,Zhongda Hospital,School of Life Sciences and Technology,Advanced Institute for Life and Health,Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research,Southeast University,Nanjing 210096,China

School of Engineering,Vanderbilt University,Nashville 37240,USA

Department of Otolaryngology-Head and Neck Surgery,Affiliated Drum Tower Hospital of Nanjing University Medical School,Jiangsu Provincial Key Medical Discipline(Laboratory),Nanjing 210096,China

Research Institute of Otorhinolaryngology,Nanjing 210096,China

Department of Neurology,Aerospace Center Hospital,School of Life Sciences,Beijing Institute of Technology,Beijing 100081,China

Advanced Technology Research Institute,Beijing Institute of Technology,Beijing 100081,China

展开 >

2024

10.1007/s11427-024-2672-1

中国科学:生命科学(英文版)
中国科学院

中国科学:生命科学(英文版)

CSTPCD
影响因子：0.806
ISSN：1674-7305
年,卷(期)：2024.67(11)