首页|Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus
Unveiling potential sex-determining genes and sex-specific markers in autotetraploid Carassius auratus
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Autotetraploid Carassius auratus is a stable hereditary autotetraploid fish resulting from the hybridization of Carassius auratus red var.(RCC,♀)× Megalobrama amblycephala(BSB,♂),containing four sets of RCC chromosomes.However,the molecular mechanism underlying the determination of sex in this species remains largely unknown.Currently,there lacks a full understanding of the molecular mechanisms governing sex determination and specific molecular markers to differentiate sex in this species.In this study,25,801,677 SNPs(Single-nucleotide polymorphism)and 6,210,306 Indels(insertion-deletion)were obtained from whole-genome resequencing of 100 individuals(including 50 female and 50 male).Further identification confirmed the candidate chromosomes as Chr46B,with the sex-determining region located at Chr46B:22,500,000-22,800,000 bp.Based on the male-specific insertion(26 bp)within the candidate sex-determining region,a pair of sex-specific molecular markers has been identified.In addition,based on the screening of candidate sex-determining region genes and RT-qPCR validation analysis,ADAM10,AQP9 and tc1a were identified as candidate sex-determining genes.These findings provide a robust foundation for investigating sex determination mechanisms in fish,the evolution of sex chromosomes,and the development of monosex populations.
Kun Zhang、Xu Huang、Chongqing Wang、Xidan Xu、Xiaowei Xu、Xiaoping Dong、Qingwen Xiao、Jinhai Bai、Yue Zhou、Zhengkun Liu、Xinyi Deng、Yan Tang、Siyang Li、Enkui Hu、Wanjing Peng、Ling Xiong、Qinbo Qin、Shaojun Liu
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State Key Laboratory of Developmental Biology of Freshwater Fish,Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry,College of Life Sciences,Hunan Normal University,Changsha 410081,China
Nansha-South China Agricultural University Fishery Research Institute,Guangzhou 511457,China
Hunan Yuelu Mountain Science and Technology Co.,Ltd.,for Aquatic Breeding,Changsha 410081,China
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