Multi-omics analysis reveals the genetic and environmental factors in shaping the gut resistome of a keystone rodent species

扫码查看

原文链接

万方数据

外文摘要：Understanding the emergence and spread of antibiotic resistance genes(ARGs)in wildlife is critical for the health of humans and animals from a"One Health"perspective.The gut microbiota serve as a reservoir for ARGs;however,it remains poorly understood how environ-mental and host genetic factors influence ARGs by affecting the gut microbiota.To elucidate this,we analyzed whole-genome resequencing data from 79 individuals of Brandt's vole in two geographic locations with different antibiotics usage,together with metabolomic data and shotgun sequencing data.A high diversity of ARGs(851 subtypes)was observed in vole's gut,with a large variation in ARG composition between individuals from Xilingol and Hulunbuir in China.The diversity and composition of ARGs were strongly correlated with variations in gut microbiota community structure.Genome-wide association studies revealed that 803 loci were significantly associated(P＜5.05x10-9)with 31 bacterial species,and bipartite networks identified 906 bacterial species-ARGs associations.Structural equation modeling analysis showed that host genetic factors,air temperature,and presence of pollutants(Bisphenol A)significantly affected gut microbiota community structure,which eventually regulated the diversity of ARGs.The present study advances our understanding of the complex host-environ-ment interactions that underlie the spread of ARGs in the natural environments.

外文关键词：

antibiotic resistanceBrandt's volegut resistomeGWAS

作者：

Guoliang Li、Dong Zhu、Chaoyuan Cheng、Haiyan Chu、Fuwen Wei、Zhibin Zhang

展开 >

作者单位：

State Key Laboratory of Integrated Management on Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China

Jiangxi Provincial Key Laboratory of Conservation Biology,College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China

CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China

Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China

State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China

展开 >

出版年：

2024

DOI：

10.1007/s11427-024-2679-3

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(11)