6-Phosphogluconate dehydrogenase 2 bridges the OPP and shikimate pathways to enhance aromatic amino acid production in plants

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外文摘要：The oxidative pentose phosphate(OPP)pathway provides metabolic intermediates for the shikimate pathway and directs carbon flow to the biosynthesis of aromatic amino acids(AAAs),which serve as basic protein building blocks and precursors of numerous metabolites essential for plant growth.However,genetic evidence linking the two pathways is largely unclear.In this study,we identified 6-phosphogluconate dehydrogenase 2(PGD2),the rate-limiting enzyme of the cytosolic OPP pathway,through suppressor screening of arogenate dehydrogenase 2(adh2)in Arabidopsis.Our data indicated that a single amino acid substitution at position 63(glutamic acid to lysine)of PGD2 enhanced its enzyme activity by facilitating the dissociation of products from the active site of PGD2,thus increasing the accumulation of AAAs and partially restoring the defective phenotype of adh2.Phylogenetic analysis indicated that the point mutation occurred in a well-conserved amino acid residue.Plants with different amino acids at this conserved site of PGDs confer diverse catalytic activities,thus exhibiting distinct AAAs producing capability.These findings uncover the genetic link between the OPP pathway and AAAs biosynthesis through PGD2.The gain-of-function point mutation of PGD2 identified here could be considered as a potential engineering target to alter the metabolic flux for the production of AAAs and downstream compounds.

外文关键词：

plant metabolismaromatic amino acidsoxidative pentose phosphate pathwayshikimate pathway6-phosphogluconate dehydrogenase

作者：

Qian Tang、Yuxin Huang、Zhuanglin Shen、Linhui Sun、Yang Gu、Huiqing He、Yanhong Chen、Jiahai Zhou、Limin Zhang、Cuihuan Zhao、Shisong Ma、Yunhai Li、Jie Wu、Qiao Zhao

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作者单位：

Shenzhen Key Laboratory of Synthetic Genomics,Guangdong Provincial Key Laboratory of Synthetic Genomics,Key Laboratory of Quantitative Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

University of Chinese Academy of Sciences,Beijing 100049,China

CAS Key Laboratory for Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Key Laboratory of Quantitative Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Key Laboratory of Agricultural Microbiology of Heilongjiang Province,Northeast Agricultural University,Harbin 150030,China

Center for Plant Biology,School of Life Sciences,Tsinghua University,Beijing 100084,China

State Key Laboratory of Plant Cell and Chromosome Engineering,CAS Centre for Excellence in Molecular Plant Biology,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China

MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics,School of Life Sciences,University of Science and Technology of China,Innovation Academy for Seed Design,Chinese Academy of Sciences,Hefei 230027,China

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出版年：

2024

DOI：

10.1007/s11427-024-2567-4

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(11)