CRISPR beyond:harnessing compact RNA-guided endonucleases for enhanced genome editing

扫码查看

原文链接

万方数据
维普

外文摘要：The CRISPR-Cas system,an adaptive immunity system in prokaryotes designed to combat phages and foreign nucleic acids,has evolved into a groundbreaking technology enabling gene knockout,large-scale gene insertion,base editing,and nucleic acid detection.Despite its transformative impact,the conventional CRISPR-Cas effectors face a significant hurdle—their size poses challenges in effective delivery into organisms and cells.Recognizing this limitation,the imperative arises for the development of compact and miniature gene editors to propel advancements in gene-editing-related therapies.Two strategies were accepted to develop compact genome editors:harnessing OMEGA(Obligate Mobile Element-guided Activity)systems,or engineering the existing CRISPR-Cas system.In this review,we focus on the advances in miniature genome editors based on both of these strategies.The objective is to unveil unprecedented opportunities in genome editing by embracing smaller,yet highly efficient genome editors,promising a future characterized by enhanced precision and adaptability in the genetic interventions.

外文关键词：

CRISPR-CasOMEGA(ω)systemtransposasegene-editingcompact editorsprotein engineeringminiature Cas effector

作者：

Feizuo Wang、Shengsheng Ma、Senfeng Zhang、Quanquan Ji、Chunyi Hu

展开 >

作者单位：

Department of Biological Sciences,Faculty of Science,National University of Singapore,Singapore 117543,Singapore

Cancer Science Institute of Singapore,National University of Singapore,Singapore 117597,Singapore

Department of Biochemistry,Yong Loo Lin School of Medicine,National University of Singapore,Singapore 117597,Singapore

Precision Medicine Translational Research Programme(TRP),Yong Loo Lin School of Medicine,National University of Singapore,Singapore 117597,Singapore

展开 >

出版年：

2024

DOI：

10.1007/s11427-023-2566-8

中国科学:生命科学(英文版)

中国科学院

中国科学:生命科学(英文版)

CSTPCD

影响因子：0.806

ISSN：1674-7305

年,卷(期)：2024.67(12)